Jedynym znakiem był napis, wyryty na palisadzie: CROATOAN, co mogło znaczyć zarówno, że fort został napadnięty, jak też, że osadnicy ewakuowali się do wioski Mantea na wyspach mierzejowych, lub zbudowali statek chcąc uciec do Anglii i zginęli gdzieś na oceanie. Wahunsenacawh, he claimed, carried out the unprovoked attack at the recommendation of his priests, shortly before the arrival of the Jamestown colonists. By the time Mace approached Hatteras, bad weather prevented them from lingering in the area.  On July 30, 1619, burgesses met at Jamestown Church as the first elected representative legislative assembly in the New World.
Soon, these patented tracts of land sprang up along the rivers.
David Beers Quinn concluded that the 1587 colonists sought to relocate to their original destination—Chesapeake Bay—using the pinnace and other small boats to transport themselves and their belongings.
If such an effort was made, the ship could have been lost with all hands at sea, accounting for the absence of both the ship and any trace of the colonists.
:xx, 89, 276, Lane's colony was troubled by a lack of supplies and poor relations with the local Native Americans. Although Spain, France, Sweden, and the Netherlands all had competing claims to the region, none of these prevented the English from becoming the first European power to colonize successfully the Mid-Atlantic coastline.
When the villagers did not produce the cup, the English decided that severe retribution was necessary in order to avoid the appearance of weakness. There are indications that Thomas Harriot and John White may have participated in the voyage, but no records survive which directly confirm their involvement. Plantations and new outposts sprung up starting with Henricus, initially both upriver and downriver along the navigable portion of the James, and thereafter along the other rivers and waterways of the area. W lipcu 1584 roku angielska wyprawa odkrywcza, prowadzona przez kapitanów Philipa Amadasa i Arthura Barlowe’a, a finansowana przez sir Waltera Raleigh’a i wspierana przez królową Elżbietę I, dotarła do łańcucha mierzejowych wysepek przybrzeżnych (ang. On August 9, 1610, tired of waiting for a response from Powhatan, West sent George Percy with 70 men to attack the Paspahegh capital, burning the houses and cutting down their cornfields. :44–55, Upon their return to England in the autumn of 1584, Amadas and Barlowe spoke highly of the tribes' hospitality and the strategic location of Roanoke. :26, 85–86, The Historie of Travaile never directly identifies the tribe that supposedly hosted the Roanoke colonists. :151, 154, By May 1609, word had reached England's Royal Council for Virginia that the 1587 colonists had been massacred by Wahunsenacawh. As of 2019, the project has yet to identify any living descendants either.
However, Opechancanough, still preferring to use Powhatan tactics, did not make any major follow-up to this attack. :129:122 The account captivated the American public. W niecałe 20 lat później, w roku 1607 powstało pierwsze stałe osiedle angielskie Jamestown na ziemi amerykańskiej.
Among the purported advantages of this location were proximity to "Riche Copper mines of Ritanoc" and "Peccarecamicke", where four of Raleigh's colonists were supposed to be held by a chieftain named "Gepanocon".
The original map is now lost, but a copy was obtained by Pedro de Zúñiga, the Spanish ambassador to England, who passed it on to King Philip III of Spain.
He advocated for England to facilitate the Powhatans' conversion to Christianity. By 1612, Rolfe's new strains of tobacco had been successfully cultivated and exported, establishing a first cash crop for export. In the meantime, Grenville established contact with local Spanish authorities, in the hopes of obtaining fresh provisions. :111, Raleigh later sought to enforce his monopoly on Virginia—based on the potential survival of the Roanoke colonists—when the price of sassafras skyrocketed. Although there are a number of theories about the fate of the colony, it remains a mystery and has come to be known as the "Lost Colony".
 Despite the resistance of the Virginia Cavaliers, Virginian Puritan Richard Bennett was made Governor answering to Cromwell in 1652, followed by two more nominal "Commonwealth Governors". :84–85 Manteo and Towaye, who had left the Lane colony with Drake's fleet, were also brought along.
 Opechancanough, variously reported to be 92 to 100 years old, was taken to Jamestown. Late the next year, the remaining 45 sailed home, and the Plymouth company fell dormant. :112, While preparing to compose a 1937 drama about the Lost Colony, Paul Green noticed that Spanish records from the period contained an abundance of references to Raleigh and his settlements. in, Spangler, Jewel L. "Becoming Baptists: Conversion in colonial and early national Virginia.
However, the colonists would quickly spread European diseases among their hosts, decimating the Chowanoke and thereby destabilizing the balance of power in the region. The group had set foot on the island on 22 nd July 1587. Pace, after securing himself and his neighbors on the south side of the James River, took a canoe across river to warn Jamestown, which narrowly escaped destruction, although there was no time to warn the other settlements.
 The leader of the undead colonists, "The Butcher", is depicted as John White's wife Thomasin, although there is no historical evidence that she was one of the colonists. Instead of personally leading voyages to the Americas, he delegated the missions to his associates and oversaw operations from London.
 During 1606, each company organized expeditions to establish settlements within the area of their rights. Ludzie Amadasa i Barlowe’a spotkali miejscowych Indian z plemienia Kroatanów i nawiązali z nimi bliskie, przyjazne kontakty. Jamestown occupied land belonging to the Powhatan Confederacy, and was also at the brink of failure before the arrival of a new group of settlers and supplies by ship in 1610. Likewise, Augusta Academy was a classical school that would evolve into Washington and Lee University (though would not grant its first bachelor's degree until 1785).
:97, The Spanish Empire had been gathering intelligence on the Roanoke colonies since Grenville's capture of Santa Maria de San Vicente in 1585. In 1640, John Punch was sentenced to lifetime servitude as punishment for trying to escape from his master Hugh Gwyn. Sir Walter Raleigh proceeded to seek investors to fund a colony. Seven of the other ships had arrived carrying more colonists, but little in the way of food and supplies. Supply ships arrived at the now-abandoned colony later in 1586; 15 soldiers were left behind to hold the island, but no trace of these men was later found. On March 25, 1584, Queen Elizabeth I issued a charter allowing Raleigh to “discover, search, find out, and view such remote heathen and barbarous Lands, Countries, and territories … to have, hold, occupy, and enjoy.”.
Wykorzystując plotkę o planowanym rzekomo ataku Indian, Lane dokonał 1 czerwca 1586 r. zdradzieckiego ataku; Wingina został zabity.
:225–26, During Raleigh's first transatlantic voyage in 1595, he claimed to be in search of his lost colonists, although he would admit this was disinformation to cover his search for El Dorado. :62 While visiting Roanoke Island itself, Lawson reported finding the remains of a fort, as well as English coins, firearms, and a powder horn.:138. Although Strachey accused Wahunsenacawh of slaughtering the colonists and Chesepians in separate passages, Quinn decided that these events occurred in a single attack on an integrated community, in April 1607.  Some Welsh arrived including the ancestors of Thomas Jefferson.. :102–10 Although much of their research did not survive the 1586 evacuation of the colony, Harriot's extensive survey of Virginia's inhabitants and natural resources was published in 1588, with engravings of White's illustrations included in the 1590 edition.
When he returned the only sign of the colonists were the letters “CRO” carved on a tree and the word “CROATOAN” carved on one of the palisade’s entrance posts.
:120–23, White dispatched Stafford to re-establish relations with the Croatan, with the help of Manteo. They arrived shortly before the English settlers, and displaced the resident Siouan tribes.  In the early 18th century, indentured German-speaking colonists from the iron-working region of Nassau-Siegen arrived to establish the Germanna settlement. :104, 108, Amadas spent the winter exploring Chesepeake Bay, travelling as far as Cape Henry and the James River. :60–63, The fleet sailed through an inlet at Wococon Island (near present-day Ocracoke Inlet) on June 26. The wall also served to contain cattle. Lost Colony) na wyspie Roanoke w stanie Karolina Północna w USA – pierwsza angielska osada na obszarze Ameryki Północnej. Raleigh sent a second expedition to Roanoke Island and appointed Ralph Lane as Governor. :12, Despite the broad powers granted to Raleigh, he was forbidden to leave the queen's side.
Grenville's ships then sailed to La Isabela, where the Spanish set aside hostilities to trade with the well-armed English fleet.
", "The fourth voyage made to Virginia with three ships, in yere 1587.
With financial backing from a number of influential shareholders, Sir Humphrey Gilbert, Walter Raleigh, and seven ships sailed from Plymouth, England in November 1578 to establish a colony in Newfoundland.
, Archaeological research on Roanoke Island only began when Talcott Williams discovered a Native American burial site in 1887. Known as Pilgrims, they successfully established a settlement in what became Massachusetts. The tribe petitioned to be renamed in 1911, eventually settling on the name Lumbee in 1956. The English called this place "Bay of Muskito" (Mosquito Bay) while the Spanish called it "Mosquetal".
The local Indians attacked the fort but the colonists were able to repel it.
The Virginia Company's two settlements, Virginia and Bermuda (Bermuda's Independent Puritans were expelled as the Eleutheran Adventurers, settling the Bahamas under William Sayle), Antigua and Barbados were conspicuous in their loyalty to the Crown, and were singled out by the Rump Parliament in An Act for prohibiting Trade with the Barbadoes, Virginia, Bermuda and Antego in October 1650.  The survivors from Bermuda had brought few supplies and food with them, and it appeared to all that Jamestown must be abandoned and it would be necessary to return to England.
:276 Both the colony and the adult Virginia character were embraced as symbols of white nationalism.
:129, 131, Smith planned to explore Pakrakanick, but a dispute with the Paspahegh ended the mission before it could begin. :181–82 Lawson was impressed with the influence of English culture on the Hatteras. Within two weeks they had constructed their first fort and named their settlement Jamestown. During his travels, Harriot and his assistants collected data that would eventually be used to produce White's La Virginea Pars map. Youth (3 - 17): $8 They told of the travelings of the colonists and their ultimate deaths. :116–25 Interest in the matter fell into decline until 1834, when George Bancroft published his account of the events in A History of the United States.
X-ray analysis in 2017 proved the ring was brass, not gold, and experts could not confirm the alleged connection to Kendall heraldry. :97–98 Lane had expected the Moratuc to provide provisions for him along his route, but Pemisapan had sent word that the English were hostile and villagers should withdraw from the river with their food. W sierpniu Manteo został nazwany Lordem Roanoke, ochrzczony pod imieniem Dasamonquepeuk i wyznaczony – jako uzurpator – na następcę wrogiego białym Wanchese’a.
It is widely accepted that the Croatan were ancestors of the 18th-century Hatteras, although evidence of this is circumstantial.